...According to a study published this Wednesday in the journal PLOS ONE, the DNA obtained from the tooth is also the oldest ever sequenced for a domesticated horse from the Americas.
The tooth was excavated from one of Spain’s first colonized settlements. Located on the island of Hispaniola, the town of Puerto Real was established in 1507 and served for decades as the last port of call for ships sailing from the Caribbean. But rampant piracy and the rise of illegal trade in the 16th century forced the Spanish to consolidate their power elsewhere on the island, and in 1578, residents were ordered to evacuate Puerto Real. The abandoned town was destroyed the following year by Spanish officials.
...Horse fossils and associated artifacts are incredibly rare at Puerto Real and similar sites from the time period, but cow remains are a common find. According to Delsol, this skewed ratio is primarily due to the way Spanish colonialists valued their livestock.
“Horses were reserved for individuals of high status, and owning one was a sign of prestige,” he said. “There are full-page descriptions of horses in the documents that chronicle the arrival of [Hernán] Cortés in Mexico, demonstrating how important they were to the Spanish.”
In contrast, cows were used as a source of meat and leather, and their bones were regularly discarded in communal waste piles called middens. But one community’s trash is an archaeologist’s treasure, as the refuse from middens often confers the clearest glimpse into what people ate and how they lived.
...The specimen’s biggest surprise wasn’t revealed until Delsol compared its DNA with that of modern horses from around the world. Given that the Spanish brought their horses from the Iberian Peninsula in southern Europe, he expected horses still living in that region would be the closest living relatives of the 500-year-old Puerto Real specimen.
Instead, Delsol found its next of kin over 1,000 miles north of Hispaniola, on the island of Assateague off the coast of Maryland and Virginia. Feral horses have roamed freely across the long stretch of barrier island for hundreds of years, but exactly how they got there has remained a mystery....